VEGFA Antikörper
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- Target Alle VEGFA Antikörper anzeigen
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser VEGFA Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Spezifität
- Human VEGF
- Aufreinigung
- Protein A purified
- Immunogen
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E.coli derived recombinant Human VEGF165.
Type of Immunogen: Cell extract - Isotyp
- IgG1
- Top Product
- Discover our top product VEGFA Primärantikörper
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- Applikationshinweise
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Approved: ELISA (2 - 4 μg/mL), WB (0.25 - 0.5 μg/mL)
Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/mL), a concentration of &le. 0.2 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with biotinylated antigen affinity purified anti-human VEGF165 (LS-C104615) as the detection antibody at a concentration of approximately 0.25-1 μg/mL. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- Andere Bezeichnung
- VEGFA / VEGF (VEGFA Produkte)
- Hintergrund
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Name/Gene ID: VEGFA
Family: PDGF
Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1 - Gen-ID
- 7422
- UniProt
- P15692
- Pathways
- RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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