IL-2 Antikörper

Produktnummer ABIN465313

Produktdetails anti-IL-2 Antikörper

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-2 Antikörper ist unkonjugiert

Applikation: ELISA

Spezifität: Human IL-2

Aufreinigung: Protein A purified

Immunogen: E. coli derived recombinant Human IL-2.

Type of Immunogen: Cell extract

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (2 - 4 μg/mL), SwELISA

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 μg/mL-5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-2 when used with biotinylated antigen affinity purified anti-human IL-2 (LS-C104522) as the detection antibody at a concentration of approximately 1-2 μg/mL. Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 1-2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 2-4 ng/lane, under reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in Sterile water to a concentration on 1 mg/mL

Konzentration: Lot specific

Buffer: Lyophilized from PBS

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: IL2 (IL2 Produkte)

Synonyme: IL2 antikoerper, IL-2 antikoerper, il2 antikoerper, TCGF antikoerper, lymphokine antikoerper, Il-2 antikoerper, POIL2 antikoerper, interleukin 2 antikoerper, interleukin-15 antikoerper, IL2 antikoerper, il2 antikoerper, LOC100303688 antikoerper, Il2 antikoerper

Hintergrund: Name/Gene ID: IL2
Family: Interleukin

Synonyms: IL2, Aldesleukin, Interleukin 2, Lymphokine, IL-2, Interleukin-2, TCGF, T cell growth factor, T-cell growth factor

Gen-ID: 3558

UniProt: P60568

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation