EGF Antikörper
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- Target Alle EGF Antikörper anzeigen
- EGF (Epidermal Growth Factor (EGF))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser EGF Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Spezifität
- Human EGF
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human EGF.
Type of Immunogen: Cell extract - Top Product
- Discover our top product EGF Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.25 - 0.35 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL-0.25 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hEGF (25 pg/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (LS-C104809) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Sterile water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- EGF (Epidermal Growth Factor (EGF))
- Andere Bezeichnung
- EGF (EGF Produkte)
- Synonyme
- HOMG4 antikoerper, URG antikoerper, AI790464 antikoerper, CEGF antikoerper, epidermal growth factor antikoerper, pro-epidermal growth factor antikoerper, EGF antikoerper, egf antikoerper, CpipJ_CPIJ020278 antikoerper, Egf antikoerper
- Hintergrund
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Name/Gene ID: EGF
Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor - Gen-ID
- 1950
- UniProt
- P01133
- Pathways
- NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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