Thrombopoietin Antikörper (Biotin)

Produktnummer ABIN465560

Produktdetails anti-Thrombopoietin Antikörper (Biotin)

Target: Thrombopoietin (THPO)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Thrombopoietin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Spezifität: Human TPO

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TPO.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hTPO by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TPO (LS-C104802) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Thrombopoietin

Target: Thrombopoietin (THPO)

Andere Bezeichnung: THPO / TPO / Thrombopoietin (THPO Produkte)

Hintergrund: Name/Gene ID: THPO
Family: Hormone

Synonyms: THPO, C-mpl ligand, MGDF, MPL ligand, THCYT1, Thrombopoietin nirs variant 1, MKCSF, MPLLG, Thrombopoietin

Gen-ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signalweg, Hormone Activity