IL-33 Antikörper
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- Target Alle IL-33 (IL33) Antikörper anzeigen
- IL-33 (IL33) (Interleukin 33 (IL33))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser IL-33 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Spezifität
- Human IL-33
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human IL-33.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL33 Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.5 - 2 μg/mL), SwELISA, WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-33 (1 ng/mL), a concentration of 0.2-0.4 μg/mL of this antibody is required. ELISA: To detect hIL-33 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-33 (LS-C104696) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-33. Western Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Sterile water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- IL-33 (IL33) (Interleukin 33 (IL33))
- Andere Bezeichnung
- IL33 (IL33 Produkte)
- Synonyme
- IL33 antikoerper, C9orf26 antikoerper, DVS27 antikoerper, IL1F11 antikoerper, NF-HEV antikoerper, NFEHEV antikoerper, RP11-575C20.2 antikoerper, 9230117N10Rik antikoerper, Il-33 antikoerper, Il1f11 antikoerper, RGD1311155 antikoerper, interleukin 33 antikoerper, IL33 antikoerper, Il33 antikoerper
- Hintergrund
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Name/Gene ID: IL33
Family: Interleukin
Synonyms: IL33, C9orf26, DVS27, DVS27-related protein, IL-1F11, IL-33, Interleukin-33, NFEHEV, NF-HEV, Interleukin-1 family member 11, NFHEV, IL1F11, Interleukin 33, RP11-575C20.2 - Gen-ID
- 90865
- UniProt
- O95760
- Pathways
- Production of Molecular Mediator of Immune Response
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