Desmoglein 1/2 Antikörper (C-Term)

Produktnummer ABIN470806

Produktdetails anti-Desmoglein 1/2 Antikörper

Target: Desmoglein 1/2 (DSG1/2)

Bindungsspezifität: C-Term

Reaktivität: Rind (Kuh), Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 1/2 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Species (tested so far) Human, bovine, rat, mouse. Recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells, does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines, bovine BMGE+H, BMGE-H, MDBK.

Aufreinigung: Protein A purified

Immunogen: Band 3 polypeptide of isolated desmosomes from bovine muzzle epidermis.

Type of Immunogen: Protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Immunohistochemistry (frozen sections, paraffin-embedded tissue after microwave treatment), Western Blot, Cryo, Paraffin (Microwave), Immunofluorescence microscopy. Immunohistochemistry: 1:10 with PBS,  pH 7.4, Incubation Time 1 h at RT, extended with paraffin.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Desmoglein 1/2

Target: Desmoglein 1/2 (DSG1/2)

Andere Bezeichnung: Desmoglein 1/2 (DSG1/DSG2) (DSG1/2 Produkte)

Synonyme: DSG1 antikoerper, desmoglein 1 antikoerper, DSG1 antikoerper

Hintergrund: This antibody detects: DSG1+2 / Desmoglein 1+2