Desmoglein 1/2 Antikörper (C-Term)
Kurzübersicht für Desmoglein 1/2 Antikörper (C-Term) (ABIN470806)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- C-Term
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Spezifität
- Species (tested so far) Human, bovine, rat, mouse. Recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells, does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines, bovine BMGE+H, BMGE-H, MDBK.
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Aufreinigung
- Protein A purified
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Immunogen
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Band 3 polypeptide of isolated desmosomes from bovine muzzle epidermis.
Type of Immunogen: Protein -
Isotyp
- IgG1
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Applikationshinweise
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Approved: IF, IHC, IHC-Fr, IHC-P, WB
Usage: Suitable for use in Immunohistochemistry (frozen sections, paraffin-embedded tissue after microwave treatment), Western Blot, Cryo, Paraffin (Microwave), Immunofluorescence microscopy. Immunohistochemistry: 1:10 with PBS, pH 7.4, Incubation Time 1 h at RT, extended with paraffin. -
Kommentare
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Target Species of Antibody: Bovine
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- deionized water
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Konzentration
- Lot specific
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Buffer
- Lyophilized.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Desmoglein 1/2 (DSG1/2)
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Andere Bezeichnung
- Desmoglein 1/2 (DSG1/DSG2)
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Hintergrund
- This antibody detects: DSG1+2 / Desmoglein 1+2
Target
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