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FHL2 Antikörper

FHL2 Reaktivität: Human, Maus WB, IHC (p), IP Wirt: Maus Monoclonal 31-10-34 unconjugated
Produktnummer ABIN487469
  • Target Alle FHL2 Antikörper anzeigen
    FHL2 (Four and A Half LIM Domains 2 (FHL2))
    Reaktivität
    • 42
    • 30
    • 17
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Maus
    Wirt
    • 34
    • 7
    • 2
    Maus
    Klonalität
    • 29
    • 13
    Monoklonal
    Konjugat
    • 25
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser FHL2 Antikörper ist unkonjugiert
    Applikation
    • 24
    • 11
    • 10
    • 8
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
    Spezifität
    This antibody reacts with Mouse FHL2.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human and Mouse.
    Produktmerkmale
    Synonyms: FHL-2, SLIM 3, DRAL, Four and a half LIM domains protein 2, Skeletal muscle LIM-protein 3,LIM domain protein DRAL
    Aufreinigung
    Protein-A Sepharose Chromatography.
    Immunogen
    His-FHL2. Remarks: Hybridoma was established by fusion of Mouse myeloma cell SP2/0-Ag4 withBalb/c mouse splenocyte.
    Klon
    31-10-34
    Isotyp
    IgG2a
    Top Product
    Discover our top product FHL2 Primärantikörper
  • Applikationshinweise
    Western blot: 1-5 μg/mLPositive Control: C2C12Immunoprecipitation: 3 μg/200-300 μL of cell extract. Positive Control: C2C12Immunohistochemistry: 1-5 μg/mLHeat treatment is necessary for Paraffin Embedded Sections. Autoclave, 10 minutes in citrate buffer ( pH 6.5) at 110 °C
    Protokoll
    SDS-PAGE and Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volumes of cold Lysis buffer (50mM Tris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the Lysisbuffer to make an 8 mg/mL solution. 3) Mix the sample with an equal volume of Laemmli's sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 µL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system. (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for specific transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at RT or overnight at 4°C. 7) Incubate the membrane with the anti-FHL2 (11-134) monoclonal antibody (1-5 µg/mL)diluted with 1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 8) Wash the membrane with PBS (5 minutes x 6 times). 9) Incubate the membrane with the 1: 10000 POD-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 10) Wash the membrane with PBS (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriatechemiluminescence reagents for 1 minute. Remove extra reagent from the membrane bydabbing with a paper towel, and seal it in plastic wrap. 12) Expose to X-ray film in a dark room for 5 minutes. Develop the film as usual. Theconditions for exposure and development may vary. Positive Controls for Western blotting: C2C12Immunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volumes of cold Lysis buffer (50mM Tris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, then
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.2 containing 50 % Glycerol without preservatives.
    Konservierungsmittel
    Without preservative
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at -20 °C.
    Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    FHL2 (Four and A Half LIM Domains 2 (FHL2))
    Andere Bezeichnung
    FHL2 / SLIM3 (FHL2 Produkte)
    Synonyme
    AAG11 antikoerper, DRAL antikoerper, FHL-2 antikoerper, SLIM-3 antikoerper, SLIM3 antikoerper, C76204 antikoerper, FHL2 antikoerper, zgc:92428 antikoerper, zgc:103466 antikoerper, four and a half LIM domains 2 antikoerper, four and a half LIM domains 2a antikoerper, four and a half LIM domains 2b antikoerper, FHL2 antikoerper, Fhl2 antikoerper, fhl2 antikoerper, fhl2a antikoerper, fhl2b antikoerper
    Hintergrund
    Proteins containing LIM domains (which are double zinc finger motifs implicated in protein binding) are important regulators of cell growth, cell differentiation, and remodeling of the cell cytoskeleton. Human Four-and-a-half LIM-only protein 2 (FHL2), also known as DRAL/Slim3 is a 32 kDa protein expressed predominantly in human heart and to a lesser extent in skeletal muscle, testis, and prostate epithelium. Since FHL2 is abundant in heart tissue, it may play a role in the regulation of myofibrillogenesis of heart via LIM-domain binding to focal adhesions. FHL2 has also been identified as a co-activator of the androgen receptor where it promotes androgen receptor transcriptional activity. Stimulation of the Rho signaling pathway induces translocation of FHL2 to the nucleus and subsequent activation of FHL2- and androgen receptor-dependent genes. FHL2 also acts as a trancriptional repressor in muscle cells and is involved in modulation of beta-catenin-dependent transcription of Wnt-responsive genes.Synonyms: DRAL, FHL-2, Four and a half LIM domains protein 2, LIM domain protein DRAL, SLIM 3, Skeletal muscle LIM-protein 3
    Gen-ID
    2274
    UniProt
    Q14192
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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