Detects ~78 kDa protein. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in Yeast, Plant, nematode and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
Kreuzreaktivität (Details)
Species reactivity (tested):Drosophila, Yeast, Saccharomyce, Plants (barnyard grass, beet, cotton, mung bean, sorghum and wheat).
Produktmerkmale
Synonyms: KDEL endoplasmic reticulum protein retention receptor 1, ERD2.1, ER marker, ER lumenprotein retaining receptor 1, Putative MAPK-activating protein PM23
Aufreinigung
Protein G Chromatography.
Immunogen
A synthetic HDEL peptide corresponding to the C-terminus of Yeast Bip.
KDELR1
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
Western blot: 1/1000-1/2000.1 μg/mL of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG -HRP asthe secondary antibody. Immunofluorescence: 1/50-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 50 % Glycerol with 0.09 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer. Shelf life: one year from despatch.
Haltbarkeit
12 months
Target
KDELR1
(KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))
HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3).Synonyms: ER lumen protein retaining receptor 1, ER marker, ERD2.1, KDEL endoplasmic reticulum protein retention receptor 1, Putative MAPK-activating protein PM23