Kanamycin Antikörper

Produktnummer ABIN487712

Produktdetails anti-Kanamycin Antikörper

Target: Kanamycin

Reaktivität: Streptomyces kanamyceticus

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Kanamycin Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Aufreinigung: Ig Fraction

Immunogen: Kanamycin-BTG

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 0.312 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10.77 mg/mL (by U.V. abs at 280nm)

Buffer: 20 mM Phosphate, 150 mM Sodium Chloride, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, store undiluted (in aliquots) at-20 °C.

Details zu Kanamycin

Target: Kanamycin

Abstract: Kanamycin Produkte

Substanzklasse: Chemical

Hintergrund: Kanamycin (also known as kanamycin A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a wide variety of infections. Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is kanamycin sulfate. Kanamycin is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for kanamycin resistance (primarily Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin (50-100 μg/mL) containing agar plates or are grown in media containing kanamycin (50-100 μg/mL). Only the bacteria that have successfully taken up the kanamycin resistance gene become resistant and will grow under these conditions. As a powder kanamycin is white to off-white and is soluble in water (50 mg/mL). Mammalian cells and other eukaryotes are screened using G418, a similar aminoglycoside antibiotic, which KanMX confers resistance against. At least one such gene, Atwbc19 is native to a plant species, of comparatively large size and its coded protein acts in a manner which decreases the possibility of horizontal gene transfer from the plant to bacteria, it may be incapable of giving resistance to kanamycin to bacteria even if gene transfer occurs.Synonyms: Kanamycin A, Kantrex