alpha Actinin 4 Antikörper (AA 561-661)

Produktnummer ABIN4886421

Produktdetails anti-alpha Actinin 4 Antikörper

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Bindungsspezifität: AA 561-661

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha Actinin 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Alpha-actinin-4(ACTN4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Alpha-actinin-4(ACTN4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: actinin alpha 4
Protein Name: Alpha-actinin-4

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Alpha Actinin 4 recombinant protein (Position: E561-V661). Human Alpha Actinin 4 shares 99% and 98% amino acid (aa) sequence identity with mouse and rat Alpha Actinin 4, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Andere Bezeichnung: ACTN4 (ACTN4 Produkte)

Synonyme: fsgs antikoerper, fsgs1 antikoerper, C77391 antikoerper, ACTININ-4 antikoerper, FSGS antikoerper, FSGS1 antikoerper, wu:fb53f05 antikoerper, zgc:63508 antikoerper, actinin alpha 4 antikoerper, actinin, alpha 4 antikoerper, actinin alpha 4 S homeolog antikoerper, actn4 antikoerper, ACTN4 antikoerper, Actn4 antikoerper, actn4.S antikoerper

Hintergrund: Alpha-actinin-4 is a protein that in humans is encoded by the ACTN4 gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This ACTN4 gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Synonyms: actinin4 | actinin-4 | actinin 4 | ACTN4 | ACTN 4 | FSGS1 | FSGS 1 | O43707

Gen-ID: 81

UniProt: O43707

Pathways: Proton Transport