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ELANE Antikörper (AA 27-265)

ELANE Reaktivität: Maus, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN4886568
  • Target Alle ELANE Antikörper anzeigen
    ELANE (Elastase, Neutrophil Expressed (ELANE))
    Bindungsspezifität
    • 10
    • 7
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 27-265
    Reaktivität
    • 70
    • 4
    • 3
    Maus, Ratte
    Wirt
    • 51
    • 16
    • 5
    • 1
    Kaninchen
    Klonalität
    • 48
    • 25
    Polyklonal
    Konjugat
    • 41
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser ELANE Antikörper ist unkonjugiert
    Applikation
    • 35
    • 34
    • 15
    • 10
    • 8
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Verwendungszweck
    Rabbit IgG polyclonal antibody for Neutrophil elastase(ELANE) detection. Tested with WB, IHC-P in Mouse,Rat.
    Kreuzreaktivität (Details)
    No cross reactivity with other proteins.
    Produktmerkmale
    Rabbit IgG polyclonal antibody for Neutrophil elastase(ELANE) detection. Tested with WB, IHC-P in Mouse,Rat.
    Gene Name: elastase, neutrophil expressed
    Protein Name: Neutrophil elastase
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265). Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2.
    Isotyp
    IgG
    Top Product
    Discover our top product ELANE Primärantikörper
  • Applikationshinweise
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Target
    ELANE (Elastase, Neutrophil Expressed (ELANE))
    Andere Bezeichnung
    ELANE (ELANE Produkte)
    Synonyme
    ELA2 antikoerper, GE antikoerper, HLE antikoerper, HNE antikoerper, NE antikoerper, PMN-E antikoerper, SCN1 antikoerper, Ela2 antikoerper, F430011M15Rik antikoerper, ela2 antikoerper, ELANE antikoerper, elastase, neutrophil expressed antikoerper, proteinase 3 antikoerper, ELANE antikoerper, Elane antikoerper, prtn3 antikoerper
    Hintergrund
    Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

    Synonyms: ELA2 | ELANE | Elastase 2 | Elastase-2 | HLE | HNE | Leukocyte elastase | Medullasin | Neutrophil elastase | PMN E | PMN elastase | SCN1 | P08246
    Gen-ID
    50701
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