Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine andisoleucine variants.
Produktmerkmale
Expected / apparent Molecular Weight of the Antigene: 82.7 / 55-60 kDa
Aufreinigung
serum
Immunogen
KLH-conjugated synthetic peptide derived from known PsaB sequences including Arabidopsis thaliana P56767, AtCg00340
Applikationshinweise
Recommended Dilution: 1 : 1000 with standard ECL (WB).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 200 µL, of sterile water.
Handhabung
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Mustila, Allahverdiyeva, Isojärvi, Aro, Eisenhut: "The bacterial-type [4Fe-4S] ferredoxin 7 has a regulatory function under photooxidative stress conditions in the cyanobacterium Synechocystis sp. PCC 6803." in: Biochimica et biophysica acta, Vol. 1837, Issue 8, pp. 1293-304, (2014) (PubMed).
Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).
Krech, Ruf, Masduki, Thiele, Bednarczyk, Albus, Tiller, Hasse, Schöttler, Bock: "The plastid genome-encoded Ycf4 protein functions as a nonessential assembly factor for photosystem I in higher plants." in: Plant physiology, Vol. 159, Issue 2, pp. 579-91, (2012) (PubMed).
AGI Code: ATCG00340 Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaB is a core protein of PSI complex. Synonymes: Photosystem I P700 chlorophyll a apoprotein A2, PSI-B