Acetylated Lysine Antikörper
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- Target Alle Acetylated Lysine Produkte
- Acetylated Lysine
- Reaktivität
- Bitte anfragen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Acetylated Lysine Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Spezifität
- The antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
- Kreuzreaktivität (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Homologie
- higher plants
- Aufreinigung
- affinity purified
- Immunogen
- acetylated KLH
- Klon
- 7F8
- Isotyp
- IgG1
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- Applikationshinweise
- 1: 100 (IHC), 1: 1000 (WB), for other assays optimal dilution has to be determined by end user
- Kommentare
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1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 %glycerol at concentration 1 mg/mL
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. - Lagerung
- -20 °C
- Informationen zur Lagerung
- store at -20°C, avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- Acetylated Lysine
- Abstract
- Acetylated Lysine Produkte
- Synonyme
- C-K-RAS antikoerper, CFC2 antikoerper, K-RAS2A antikoerper, K-RAS2B antikoerper, K-RAS4A antikoerper, K-RAS4B antikoerper, KI-RAS antikoerper, KRAS1 antikoerper, KRAS2 antikoerper, NS antikoerper, NS3 antikoerper, RASK2 antikoerper, KRAS proto-oncogene, GTPase antikoerper, KRAS antikoerper
- Substanzklasse
- Chemical
- Hintergrund
- Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
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