Species reactivity (tested):Mouse, Rat, Bovine, Human.
Produktmerkmale
Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-IIsubunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain
CAMK2A
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
RIA. ELISA. Immunflourescence. Immunoprecipitation. Immunohistochemistry. Western blot: 0.1 μg/mL of this CamKIIA antibody was needed for detection of CaMKII in 20 μg of Rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-MouseIgG: AP as the secondary antibody.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Haltbarkeit
12 months
Target
CAMK2A
(Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
camk2 antikoerper, camk2b antikoerper, zgc:112538 antikoerper, zgc:123320 antikoerper, CAMKA antikoerper, CaMKII antikoerper, R74975 antikoerper, mKIAA0968 antikoerper, PK2CDD antikoerper, PKCCD antikoerper, calcium/calmodulin dependent protein kinase II alpha antikoerper, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antikoerper, calcium/calmodulin-dependent protein kinase II alpha antikoerper, CAMK2A antikoerper, camk2a.S antikoerper, camk2a antikoerper, Camk2a antikoerper
Hintergrund
CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca 2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968