EIF2AK2 Antikörper (AA 519-550)

Produktnummer ABIN3030885

Produktdetails anti-EIF2AK2 Antikörper

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Bindungsspezifität: AA 519-550

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF2AK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Aufreinigung: Purified

Immunogen: A portion of amino acids 519-550 from the human protein was used as the immunogen for this PRKR antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the PRKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PRKR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Andere Bezeichnung: PRKR (EIF2AK2 Produkte)

Synonyme: PKR antikoerper, pkr antikoerper, EIF2AK2 antikoerper, DKFZp469K1934 antikoerper, EIF2AK1 antikoerper, PRKR antikoerper, 2310047A08Rik antikoerper, 4732414G15Rik antikoerper, AI467567 antikoerper, AI747578 antikoerper, Pkr antikoerper, Prkr antikoerper, Tik antikoerper, eukaryotic translation initiation factor 2 alpha kinase 2 antikoerper, eukaryotic translation initiation factor 2-alpha kinase 2 antikoerper, EIF2AK2 antikoerper, eif2ak2 antikoerper, Eif2ak2 antikoerper

Hintergrund: Interferon-induced, double-stranded RNA-activated protein kinase is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation. IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region. The HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PRKR and the translation initiation factor EIF2-alpha, a target of PRKR. E2 inhibits the kinase activity of PRKR and blocks its inhibitory effect on protein synthesis and cell growth, which provides one mechanism by which HCV may circumvent the antiviral effect of interferon.

UniProt: P19525

Pathways: DNA Reparatur, ER-Nucleus Signaling, Hepatitis C