Rekombinanter 5-Hydroxymethylcytosine Antikörper

Produktnummer ABIN4953286

Produktdetails anti-5-Hydroxymethylcytosine Antikörper

Target: 5-Hydroxymethylcytosine (5-hmC)

Antikörpertyp: Recombinant Antibody

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser 5-Hydroxymethylcytosine Antikörper ist unkonjugiert

Applikation: ELISA, Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Produktmerkmale: This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

Aufreinigung: Protein A purified from animal origin-free supernatant

Immunogen: BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.

Klon: RM236

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Dot Blot: 0.2-1 μg/mL,ELISA: 0.1-1 μg/mL,Immunohistochemistry: 0.1-1 μg/mL (1),Immunocytochemistry: 0.5-2 μg/mL,hMeDIP: 0.2 μg/mL- 2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the recombinant 5hmC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Details zu 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Andere Bezeichnung: 5-Hydroxymethylcytosine (5hmC) (5-hmC Produkte)

Substanzklasse: Chemical

Hintergrund: This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.