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Rekombinanter 5-Hydroxymethylcytosine Antikörper

5-hmC ELISA, DB, hmeDIP, ICC, IHC Wirt: Kaninchen Monoclonal RM236 unconjugated Recombinant Antibody
Produktnummer ABIN4953286
  • Target Alle 5-Hydroxymethylcytosine (5-hmC) Produkte
    5-Hydroxymethylcytosine (5-hmC)
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    Bitte anfragen
    Wirt
    • 11
    • 5
    Kaninchen
    Klonalität
    • 10
    • 6
    Monoklonal
    Konjugat
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser 5-Hydroxymethylcytosine Antikörper ist unkonjugiert
    Applikation
    • 8
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    Produktmerkmale
    This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
    Aufreinigung
    Protein A purified from animal origin-free supernatant
    Immunogen
    BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.
    Klon
    RM236
    Isotyp
    IgG
  • Applikationshinweise
    The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

    1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Dot Blot: 0.2-1 μg/mL,ELISA: 0.1-1 μg/mL,Immunohistochemistry: 0.1-1 μg/mL (1),Immunocytochemistry: 0.5-2 μg/mL,hMeDIP: 0.2 μg/mL- 2 μg/mL

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store the recombinant 5hmC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
  • Target
    5-Hydroxymethylcytosine (5-hmC)
    Andere Bezeichnung
    5-Hydroxymethylcytosine (5hmC) (5-hmC Produkte)
    Substanzklasse
    Chemical
    Hintergrund
    This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
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