Dieser Alkaline Phosphatase Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
Aufreinigung
Antigen affinity purified
Immunogen
A portion of amino acids 274-304 from human ALPI was used as the immunogen for this anti-Alkaline Phosphatase antibody. This sequence is 87% homologous in human ALPP.
ALP
Reaktivität: Human
ELISA, IEM
Wirt: Schaf
Polyclonal
unconjugated
Applikationshinweise
Titration of the anti-Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-100,Immunofluorescence: 1:100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot the anti-Alkaline Phosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.