HDAC1 Antikörper (AA 449-482)

Produktnummer ABIN3031222

Produktdetails anti-HDAC1 Antikörper

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Bindungsspezifität: AA 449-482

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HDAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Kreuzreaktivität (Details): Expected species reactivity: Mouse

Aufreinigung: Purified

Immunogen: A portion of amino acids 449-482 from the human protein was used as the immunogen for this HDAC1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the HDAC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000,Immunofluorescence: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the HDAC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Andere Bezeichnung: HDAC1 (HDAC1 Produkte)

Hintergrund: HDAC1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation. [UniProt]

UniProt: Q13547

Pathways: Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis