Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
Kreuzreaktivität (Details)
Expected species reactivity: Bovine
Aufreinigung
Antigen affinity purified
Immunogen
This SMAD1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 163-196 amino acids from the Central region of human SMAD1.
SMAD1
Reaktivität: Human
WB, ELISA
Wirt: Maus
Monoclonal
2A1
unconjugated
Applikationshinweise
Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:25,IHC (Paraffin): 1:25,Western blot: 1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot the SMAD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
SMAD1
(SMAD, Mothers Against DPP Homolog 1 (SMAD1))
SMADs are intracellular proteins that transduce extracellular signals from transforming growth factor beta ligands to the nucleus where they activate downstream gene transcription. The SMADs, which form a trimer of two receptor-regulated SMADs and one co-SMAD, act as transcription factors that regulate the expression of certain genes. There are three classes of SMADs, SMAD1 belongs to the receptor-regulated class (R-SMAD) which includes SMAD2, SMAD3, SMAD5 and SMAD8/9. [Wiki]