SMAD4 Antikörper (AA 400-428)

Produktnummer ABIN3032608

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SMAD4 in WB, ELISA, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SMAD4 Antikörper (AA 400-428) (ABIN3032608)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-SMAD4 Antikörper

Bindungsspezifität: AA 400-428

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Rat, Bovine, Pig

Aufreinigung: Purified

Immunogen: A portion of amino acids 400-428 from the human protein was used as the immunogen for this SMAD4 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: SMAD4

Hintergrund: In muscle physiology, SMAD4 plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). [UniProt]

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie