SUMO4 Antikörper (Met55Val-Mutant)

Produktnummer ABIN3032655

Produktdetails anti-SUMO4 Antikörper

Target: SUMO4 (Small Ubiquitin Related Modifier 4 (SUMO4))

Bindungsspezifität: Met55Val-Mutant

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SUMO4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Aufreinigung: Antigen affinity purified

Immunogen: This SUMO4 antibody was produced from rabbits immunized with a KLH conjugated peptide CEPRGLS(V)KQIRFRFG selected from human SUMO4. This antibody is affinity purified using peptides CEPRGLS(V)KQIRFRFG (positive selection) and CEPRGLS(M)KQIRFRFG (negative selection).

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the SUMO4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the SUMO4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu SUMO4

Target: SUMO4 (Small Ubiquitin Related Modifier 4 (SUMO4))

Andere Bezeichnung: SUMO4 (SUMO4 Produkte)

Synonyme: IDDM5 antikoerper, SMT3H4 antikoerper, SUMO-4 antikoerper, dJ281H8.4 antikoerper, small ubiquitin-like modifier 4 antikoerper, small ubiquitin-related modifier 4 antikoerper, SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae) antikoerper, SUMO4 antikoerper, sumo4 antikoerper

Hintergrund: SUMO4 is a member of the SUMO gene family. This family of small ubiquitin-related modifiers covalently modify target lysines in proteins and control the target proteins' subcellular localization, stability, or activity. Upon oxidative stress, SUMO4 conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. This protein may also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to SUMO4 substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. In contrast to SUMO1, SUMO2 and SUMO3, SUMO4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. SUMO4 is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. The M55V substitution has been associated with type I diabetes.

UniProt: Q6EEV6