POU3F3 Antikörper (Internal Region)

Produktnummer ABIN498034

Produktdetails anti-POU3F3 Antikörper

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POU3F3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequenz: C-HMLSHAHQWV TAL

Spezifität: Recognizes POU3F3 / BRN1 / OCT8.

Kreuzreaktivität (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006227.1. Genename: POU3F3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/4000. Western blot: 1-3 μg/mL. Approx 48 kDa band observed in Mouse and Rat Spinal Cordlysates (calculated MW of 50.8 kDa according to Human NP_006227.1 and 50.8 kDaaccording to Mouse NP_032926.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu POU3F3

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Andere Bezeichnung: POU3F3 (POU3F3 Produkte)

Hintergrund: POU3F3, also known as Brn1, is a member of the POU family of transcription factors that is predominantly expressed in the brain. It contains a characteristic POU domain and a homeobox DNA-binding domain. POU3F3 plays a role in the development of the brain and kidney by upregulating transcription of key genes in these tissues. For example, POU3F3 interacts with the proneural protein Mash1 and POU3F2 (Brn2) on the promoter of the Notch ligand Delta1 to activate Delta1 transcription, a key step in neurogenesis.Synonyms: BRN1, Brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, OTF-8, OTF8, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, POU domain class 3 transcription factor 3

Gen-ID: 5455

NCBI Accession: NP_006227

UniProt: P20264