Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
This antibody detects endogenous levels of RFA2 protein.
Kreuzreaktivität (Details)
Species reactivity (expected):Mouse. Species reactivity (tested):Human.
Aufreinigung
Affinity chromatography using epitope-specific immunogen
Reinheit
> 95 % (by SDS-PAGE)
Immunogen
synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.
Western blot: 1: 500 - 1: 1000. immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This phospho specific polyclonal antibody is specific for phosphorylated pS122 of yeast RFA2. Reactivity with non-phosphorylated yeast RFA2 is minimal by ELISA. Immunogen: synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit