This antibody detects Decorin (Internal). It is expected to recognize the three reported isoforms a, b and c (NP_001911.1, NP_598011.1, NP_598012.1 resp.). Reported variants NP_598010.1 and NP_001911.1 represent identical protein.
Kreuzreaktivität (Details)
Species reactivity (expected):Cow, pig, caviaSpecies reactivity (tested):Human
Aufreinigung
Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Immunogen
Peptide from the internal region of the protein sequence according to NP_001911.1, NP_598011.1, NP_598012.1
DCN
Reaktivität: Human, Maus, Ratte
IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Peptide ELISA: limit dilution 1: 8000. Western blot: 0.5-2 μg/mL. Approx 38 kDa band observed in Human Kidney lysates(calculated MW of 39.8 kDa according to NP_001911.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.
Decorin is a member of the small, leucine rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF beta. It is believed that decorin also plays a role in the cell cycle of chondrocytes.Synonyms: Bone proteoglycan II, DCN, PG-S2, PG40, SLRR1B