PTRH2 Antikörper (N-Term)

Produktnummer ABIN499471

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PTRH2 in WB, IF und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PTRH2 Antikörper (N-Term) (ABIN499471)

Target: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTRH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-PTRH2 Antikörper

Bindungsspezifität: N-Term

Spezifität: Bit1 antibody was raised against a 15 amino acid peptide from near the amino-terminus of human Bit1.

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (N-Terminus) Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: Bit1 antibody can be used for the detection of Bit1 at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu PTRH2

Target: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Andere Bezeichnung: PTRH2 / BIT1

Hintergrund: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).Synonyms: CGI-147, PTH2, Peptidyl-tRNA hydrolase 2 mitochondrial

Gen-ID: 51651

NCBI Accession: NP_057161

UniProt: Q9Y3E5