Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
Spezifität
CARD9 antibody was raised against a synthetic peptide corresponding to amino acids 521 to 536 of human CARD9 The sequence is different from that of rat origin by two amino acids.
Aufreinigung
Affinity chromatography purified via peptide column
ELISA. Western Blot: CARD9 antibody can be used for detection of CARD9 at 0.5 to 1 μg/mL. A bandat approximately 59 kDa can be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C.
Target
CARD9
(Caspase Recruitment Domain Family, Member 9 (CARD9))
Gm782 antikoerper, card9 antikoerper, CANDF2 antikoerper, hCARD9 antikoerper, caspase recruitment domain family, member 9 antikoerper, caspase recruitment domain family member 9 antikoerper, Card9 antikoerper, CARD9 antikoerper, card9 antikoerper
Hintergrund
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10 (1). CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kappaB. CARD9 is an upstream activator of BCL-10 and NF-kappaB signaling.Synonyms: Caspase recruitment domain-containing protein 9