Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)
Spezifität
This antibody detects MTBP at center.
Kreuzreaktivität (Details)
Species reactivity (tested):Human
Aufreinigung
Peptide affinity chromatography
Immunogen
MTBP antibody was raised against a synthetic peptide corresponding to amino acids 122 to 139 of human MTBP, which differ from the mouse sequence by three amino acids (7).
MTBP
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
HRP
Applikationshinweise
ELISA. Western blot. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS containing 0.02 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6) and MTBP (7), were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53 (7). MTBP is expressed in a variety of normal tissues (7).Synonyms: Mdm2, Mdm2-binding protein, hMTBP