ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS containing 0.02 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Target
PIGY
(Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))
PIG-Y antikoerper, pig-y antikoerper, Prey antikoerper, Pyurf antikoerper, prey antikoerper, 1810008A14Rik antikoerper, Pigy antikoerper, phosphatidylinositol glycan anchor biosynthesis class Y antikoerper, phosphatidylinositol glycan anchor biosynthesis, class Y antikoerper, Pigy upstream reading frame S homeolog antikoerper, phosphatidylinositol glycan anchor biosynthesis, class Y-like antikoerper, PIGY antikoerper, pigy antikoerper, Pigy antikoerper, pyurf.S antikoerper, Pigyl antikoerper
Hintergrund
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.Synonyms: PIG-Y, Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein