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ISR-alpha Antikörper (AA 701-760) (AbBy Fluor® 680)

ISR-alpha Reaktivität: Human, Ratte IF (cc), IF (p), WB, FACS Wirt: Kaninchen Polyclonal AbBy Fluor® 680
Produktnummer ABIN5005448
  • Target Alle ISR-alpha Antikörper anzeigen
    ISR-alpha (Insulin Receptor alpha (ISR-alpha))
    Bindungsspezifität
    • 15
    • 15
    • 2
    • 2
    • 1
    AA 701-760
    Reaktivität
    • 52
    • 41
    • 11
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Ratte
    Wirt
    • 45
    • 7
    Kaninchen
    Klonalität
    • 33
    • 16
    • 3
    Polyklonal
    Konjugat
    • 15
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ISR-alpha Antikörper ist konjugiert mit AbBy Fluor® 680
    Applikation
    • 26
    • 25
    • 18
    • 14
    • 12
    • 11
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Western Blotting (WB), Flow Cytometry (FACS)
    Kreuzreaktivität
    Human, Ratte
    Homologie
    Mouse,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Insulin Receptor alpha
    Isotyp
    IgG
    Top Product
    Discover our top product ISR-alpha Primärantikörper
  • Applikationshinweise
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    ISR-alpha (Insulin Receptor alpha (ISR-alpha))
    Andere Bezeichnung
    Insulin Receptor alpha (ISR-alpha Produkte)
    Synonyme
    CD220 antikoerper, HHF5 antikoerper, insulin receptor antikoerper, INSR antikoerper
    Hintergrund

    Synonyms: HHF5, CD220, Insulin receptor, IR, INSR, Insulin receptor subunit alpha, PRO_0000016687

    Background: Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD, regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway.

    Gen-ID
    3643
    UniProt
    P06213
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