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beta Actin Antikörper (Middle Region)

Name: beta Actin Antikörper (Middle Region)
SKU: ABIN501930
Price: 150.78 EUR
Availability: InStock

ACTB Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Schaf, Zebrafisch (Danio rerio), Pferd, Ziege WB, IHC Wirt: Kaninchen Polyclonal unconjugated

WB Suggested Anti-ACTB Antibody Positive Control: Lane 1: 341 µg mouse 3T3 ECM extract + blocking peptide, Lane 2: 041 µg mouse 3T3 ECM extract, Lane 3: 041 µg Echinococcus granulosus extract Primary Antibody Dilution: 1:000Secondary Antibody: Anti-rabbit-HRP Secondry Antibody Dilution: 1:00,000Submitted by: Anonymous (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5. (beta Actin Antikörper (Middle Region))

Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody Secondary Antibody: Alexa goat anti-rabbit 594 Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences (beta Actin Antikörper (Middle Region))

IHC

Sample type: 1-8. (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Adult Placenta Antibody Dilution: 1. (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTBM Sample Type: Fetal Lung lysates Antibody Dilution: 1ug/ml (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: MCF7 Antibody Dilution: 1. (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1. (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Fetal Lung Antibody Dilution: 1. (beta Actin Antikörper (Middle Region))

WB Suggested Anti-ACTB Antibody Titration: 0. (beta Actin Antikörper (Middle Region))

Host: Mouse Target Name: ACTG1 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Hela Antibody Dilution: 1. (beta Actin Antikörper (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Human HepG2 Antibody Dilution: 1. (beta Actin Antikörper (Middle Region))

Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:1000 Color/Signal Descriptions : ACTB: Red DAPI:Blue Gene Name : ACTB Submitted by : Dr. (beta Actin Antikörper (Middle Region))

IHC

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5. (beta Actin Antikörper (Middle Region))

IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml. (beta Actin Antikörper (Middle Region))

IHC

Host: Rabbit Target Name: ACTB Sample Type: Jurkat Antibody Dilution: 1. (beta Actin Antikörper (Middle Region))

Rabbit Anti-ACTB Antibody Formalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (beta Actin Antikörper (Middle Region))

IHC

20 Abbildungen

(2 Referenzen)

Produktnummer ABIN501930

Datenblatt (PDF)

Target Alle beta Actin (ACTB) Antikörper anzeigen

beta Actin (ACTB) (Actin, beta (ACTB))
Bindungsspezifität
39

16

14

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Middle Region
Reaktivität
176

126

107

55

43

41

37

25

23

22

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Human, Maus, Ratte, Schwein, Rind (Kuh), Schaf, Zebrafisch (Danio rerio), Pferd, Ziege
Wirt
113

78

5

1

1
Kaninchen
Passende Sekundärantikörper
Klonalität
111

85
Polyklonal
Konjugat
124

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser beta Actin Antikörper ist unkonjugiert
Applikation
164

69

51

46

35

31

26

22

13

13

8

6

6

5

5

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz

TMKIKIIAPP ERKYSVWIGG SILASLSTFQ QMWISKQEYD ESGPSIVHRK

Homologie

Cow: 100%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Produktmerkmale

This is a rabbit polyclonal antibody against ACTB. It was validated on Western Blot.

Aufreinigung

Affinity Purified

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of Human ACTB

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ACTB Reaktivität: Human, Maus, Ratte WB, IF, IHC (p), FACS, DNA Mic Wirt: Maus Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh) WB, IHC (p) Wirt: Maus Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reaktivität: Human WB, ELISA, IHC (p) Wirt: Maus Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reaktivität: Human, Maus, Huhn, Kaninchen, Schwein, Rind (Kuh), Hamster, Ziege, Fisch WB, ELISA Wirt: Maus Monoclonal 2D1D10 unconjugated

Applikationshinweise

Optimal working dilutions should be determined experimentally by the investigator.

Kommentare

Antigen size: 375 AA

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

Lot specific

Buffer

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and may contain up to 2 % sucrose.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Avoid repeated freeze-thaw cycles.

Lagerung

-20 °C

Informationen zur Lagerung

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Phungphong, Kijtawornrat, Wattanapermpool, Bupha-Intr: "Regular exercise modulates cardiac mast cell activation in ovariectomized rats." in: The journal of physiological sciences : JPS, Vol. 66, Issue 2, pp. 165-73, (2016) (PubMed).

Rinne, Clements, Lamme, Duijf, Bolat, Meijer, Scheffer, Rosser, Tan, McGrath, Schalkwijk, Brunner, Zhou, van Bokhoven: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, (2008) (PubMed).
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung

ACTB (ACTB Produkte)

Hintergrund

This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Alias Symbols: BRWS1, PS1TP5BP1
Protein Interaction Partner: FUS, DSTN, CAP2, CFL2, CFL1, ACTG1, ACTB, PA2G4, EHHADH, WDYHV1, HDAC6, ISG15, TP53, CEP250, TUBGCP2, TUBGCP3, VCP, UBC, TUBG1, SUMO2, LGALS3BP, AMOT, NEDD1, HAUS1, IVNS1ABP, STAU1, MDM2, CORO7, LGR4, ASB12, ASB9, ZBTB1, ERG, RPA3, RPA2, RPA1, CDKN2A, PAR
Protein Size: 375

Molekulargewicht

42 kDa

Gen-ID

60

NCBI Accession

NM_001101, NP_001092

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction