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Acrolein Antikörper (Atto 488)

ACR ELISA, FACS, WB, ICC, IF, IHC Wirt: Maus Monoclonal 10A10 Atto 488
Produktnummer ABIN5067183
  • Target Alle Acrolein (ACR) Produkte
    Acrolein (ACR)
    Wirt
    • 9
    Maus
    Klonalität
    • 9
    Monoklonal
    Konjugat
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Acrolein Antikörper ist konjugiert mit Atto 488
    Applikation
    ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Spezifität
    Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.
    Aufreinigung
    Protein G Purified
    Immunogen
    Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
    Klon
    10A10
    Isotyp
    IgG1
  • Applikationshinweise
    • WB (1:1000)
    • ICC/IF (1:50)
    • FACS (1:50)
    • FCM (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    A 1:1000 dilution of ABIN5067183 was sufficient for detection of Acrolein in 2 μg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    Acrolein (ACR)
    Andere Bezeichnung
    Acrolein (ACR Produkte)
    Substanzklasse
    Chemical
    Hintergrund
    Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Acrolein is an electrophilic conjugated aldehyde that is a terminal product of lipid peroxidation. Acrolein is highly mutagenic and reacts with nucleophilic functional groups in DNA and proteins such as cysteine, histidine, and lysine residues (1).
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