HNE Antikörper (Biotin)
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- Target Alle HNE Antikörper anzeigen
- HNE (4-Hydroxynonenal (HNE))
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser HNE Antikörper ist konjugiert mit Biotin
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Applikation
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- Spezifität
- Specific for 4-HNE modified proteins. Does not detect free 4-Hydroxynonenal. Does not X-react with 4-HHE, Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
- Aufreinigung
- Protein G Purified
- Immunogen
- Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
- Klon
- 12F7
- Isotyp
- IgG1
- Top Product
- Discover our top product HNE Primärantikörper
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anti-4-Hydroxynonenal (HNE) antibody
HNE WB, ELISA, IF (p), IHC (fro), IHC (p) Wirt: Kaninchen Polyclonal unconjugated
anti-4-Hydroxynonenal (HNE) antibodyHNE WB, ELISA, IHC Wirt: Maus Monoclonal HNEJ-2 unconjugated
anti-4-Hydroxynonenal (HNE) antibodyHNE WB, ELISA, ICC, IF Wirt: Maus Monoclonal 12F7 unconjugated
anti-4-Hydroxynonenal (HNE) antibody (HRP)HNE WB, ELISA, ICC, IF Wirt: Maus Monoclonal 12F7 HRP
anti-4-Hydroxynonenal (HNE) antibody (FITC)HNE WB, ELISA, ICC, IF Wirt: Maus Monoclonal 12F7 FITC
anti-4-Hydroxynonenal (HNE) antibody (PE)HNE WB, ELISA, ICC, IF Wirt: Maus Monoclonal 12F7 PE
anti-4-Hydroxynonenal (HNE) antibody (PerCP)HNE WB, ELISA, ICC, IF Wirt: Maus Monoclonal 12F7 PerCP
anti-4-Hydroxynonenal (HNE) antibody (APC)HNE WB, ELISA, ICC, IF Wirt: Maus Monoclonal 12F7 APC
anti-4-Hydroxynonenal (HNE) antibody (Atto 594)HNE WB, ELISA, ICC, IF Wirt: Maus Monoclonal 12F7 Atto 594
anti-4-Hydroxynonenal (HNE) antibody (Atto 488)HNE WB, ELISA, ICC, IF Wirt: Maus Monoclonal 12F7 Atto 488
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- Applikationshinweise
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Kommentare
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A 1:1000 dilution of ABIN5067300 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Conjugated antibodies should be stored at 4°C
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- Target
- HNE (4-Hydroxynonenal (HNE))
- Andere Bezeichnung
- 4-Hydroxynonenal (HNE Produkte)
- Substanzklasse
- Chemical
- Hintergrund
- 4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
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