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Dityrosine Antikörper (Atto 390)

DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 Atto 390
Produktnummer ABIN5067470
  • Target Alle Dityrosine (DT) Produkte
    Dityrosine (DT)
    Wirt
    • 11
    Maus
    Klonalität
    • 11
    Monoklonal
    Konjugat
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Dityrosine Antikörper ist konjugiert mit Atto 390
    Applikation
    • 11
    • 10
    • 9
    • 9
    • 9
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Spezifität
    Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
    Aufreinigung
    Protein G Purified
    Immunogen
    Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
    Klon
    10A6
    Isotyp
    IgG1
  • Applikationshinweise
    • WB (1:1000)
    • ICC/IF (1:50)
    • FACS (1:50)
    • FCM (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    A 1:1000 dilution of ABIN5067470 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    Dityrosine (DT)
    Andere Bezeichnung
    Dityrosine (DT Produkte)
    Substanzklasse
    Dipeptide
    Hintergrund
    ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
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