Dityrosine Antikörper (Biotin)
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- Target Alle Dityrosine (DT) Produkte
- Dityrosine (DT)
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Dityrosine Antikörper ist konjugiert mit Biotin
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Applikation
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Spezifität
- Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
- Aufreinigung
- Protein G Purified
- Immunogen
- Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
- Klon
- 10A6
- Isotyp
- IgG1
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anti-Dityrosine (DT) antibody (APC)
DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 APC
anti-Dityrosine (DT) antibodyDT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 unconjugated
anti-Dityrosine (DT) antibody (HRP)DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 HRP
anti-Dityrosine (DT) antibody (FITC)DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 FITC
anti-Dityrosine (DT) antibody (PE)DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 PE
anti-Dityrosine (DT) antibody (PerCP)DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 PerCP
anti-Dityrosine (DT) antibody (Atto 594)DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 Atto 594
anti-Dityrosine (DT) antibody (Atto 488)DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 Atto 488
anti-Dityrosine (DT) antibody (Atto 390)DT WB, ELISA, ICC, IF, FACS Wirt: Maus Monoclonal 10A6 Atto 390
anti-Dityrosine (DT) antibodyDT WB, ELISA, IHC Wirt: Maus Monoclonal 1C3 unconjugated
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- Applikationshinweise
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- WB (1:1000)
- ICC/IF (1:50)
- FACS (1:50)
- FCM (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Kommentare
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A 1:1000 dilution of ABIN5067480 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Conjugated antibodies should be stored at 4°C
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- Target
- Dityrosine (DT)
- Andere Bezeichnung
- Dityrosine (DT Produkte)
- Substanzklasse
- Dipeptide
- Hintergrund
- ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
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