CD16 Antikörper

Produktnummer ABIN509571

Produktdetails anti-CD16 Antikörper

Target: CD16

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD16 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils). Regarding CD16-158V/F polymorphism, the antibody 3G8 detects both 158V and 158F allotype on natural NK cells.

Kreuzreaktivität (Details): Human, Non-Human Primates

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Human neutrophils

Klon: 3G8

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 6 μg/mL.
Immunohistochemistry (frozen sections): Acetone fixation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD16 Antikörper (ABIN509571)

Choi, Wang, Peterson, Letvin, Reimann: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." in: Immunology, Vol. 124, Issue 2, pp. 215-22, (2008) (PubMed).

Congy-Jolivet, Bolzec, Ternant, Ohresser, Watier, Thibault: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, (2008) (PubMed).

Wijngaarden, van Roon, van de Winkel, Bijlsma, Lafeber: "Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment." in: Rheumatology (Oxford, England), Vol. 44, Issue 6, pp. 729-34, (2005) (PubMed).

Metes, Ernst, Chambers, Sulica, Herberman, Morel: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." in: Blood, Vol. 91, Issue 7, pp. 2369-80, (1998) (PubMed).

Details zu CD16

Target: CD16

Andere Bezeichnung: CD16 (CD16 Produkte)

Synonyme: Fcgr3 antikoerper, Fcgr3a antikoerper, CD16 antikoerper, FCGR3 antikoerper, FCGR3@ antikoerper, CD16b antikoerper, FCG3 antikoerper, FCR-10 antikoerper, FCRIII antikoerper, FCRIIIb antikoerper, FcRIII antikoerper, Fc fragment of IgG, low affinity IIa, receptor antikoerper, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antikoerper, Fc fragment of IgG receptor IIIb antikoerper, Fc fragment of IgG receptor IIIa antikoerper, Fc receptor, IgG, low affinity III antikoerper, Fc-gamma RIII antikoerper, Fcgr2a antikoerper, FCGR3A antikoerper, FCGR3B antikoerper, Fcgr3 antikoerper, FCGR3 antikoerper

Hintergrund: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process