beta Amyloid Antikörper (C-Term)

Produktnummer ABIN5508784

Produktdetails anti-beta Amyloid Antikörper

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Amyloid Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Spezifität: A-beta 1-40, A-beta 1-42, A-beta 1-43 and APP

Kreuzreaktivität (Details): Human, mouse, rat (other species not tested)

Aufreinigung: Protein G affinity purification

Immunogen: Synthetic peptide derived from Human Abeta 1-42 protein

Klon: 9E8-1A3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1:40,000, WB: 1:4,000, IF: 1:2,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC)

Validierung #104425 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104425

Datum: 12.05.2022

Antigen: beta Amyloid

Chargennummer: 190522A

Validierte Anwendung: Immunohistochemistry

Positivkontrolle:

Adult (24 months) mouse brain

3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde

Negativkontrolle:

We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

Bewertung:

The beta amyloid antibody (Amyloid beta) (C-Term) ABIN5508784 works in IHC-P, especially at higher concentrations (1:200 and 1:500), and without the use of any antigen retrieval treatment.

Validierungsbilder

Staining of beta-amyloid positive cells in the adult mouse brain using ABIN5508784 at 40x magnification.

Protokoll

Primärantikörper: ABIN5508784

Sekundärantikörper: goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)

Full Protocol:

• Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
• Wash, dehydrate, and embed samples in paraffin wax.
• Wash several times with 0.01 M PBS.
• Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
• After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
• Incubate sections with primary anti-beta amyloid (C-Term) (antibodies Online, ABIN5508784, lot 190522A) diluted 1:200, 1:500, 1:1000, and 1:2000 in PBS-BSA-PLL ON at RT.
• Wash sections 3x 5 min with 0.01 M PBS.
• Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
• Wash sections 3x 5 min with 0.01M PBS.
• Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
• Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.

Anmerkungen:

Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min(95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4

Konservierungsmittel: Without preservative

Handhabung: Avoid freezing/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C to -80°C.

Referenzen für anti-beta Amyloid Antikörper (ABIN5508784)

Ordóñez-Gutiérrez, Posado-Fernández, Ahmadvand, Lettiero, Wu, Antón, Flores, Moghimi, Wandosell: "ImmunoPEGliposome-mediated reduction of blood and brain amyloid levels in a mouse model of Alzheimer's disease is restricted to aged animals." in: Biomaterials, Vol. 112, pp. 141-152, (2016) (PubMed).

Markoutsa, Papadia, Clemente, Flores, Antimisiaris: "Anti-Aβ-MAb and dually decorated nanoliposomes: effect of Aβ1-42 peptides on interaction with hCMEC/D3 cells." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 81, Issue 1, pp. 49-56, (2012) (PubMed).

Le Droumaguet, Nicolas, Brambilla, Mura, Maksimenko, De Kimpe, Salvati, Zona, Airoldi, Canovi, Gobbi, Magali, La Ferla, Nicotra, Scheper, Flores, Masserini, Andrieux, Couvreur: "Versatile and efficient targeting using a single nanoparticulate platform: application to cancer and Alzheimer's disease." in: ACS nano, Vol. 6, Issue 7, pp. 5866-79, (2012) (PubMed).

Canovi, Markoutsa, Lazar, Pampalakis, Clemente, Re, Sesana, Masserini, Salmona, Duyckaerts, Flores, Gobbi, Antimisiaris: "The binding affinity of anti-Aβ1-42 MAb-decorated nanoliposomes to Aβ1-42 peptides in vitro and to amyloid deposits in post-mortem tissue." in: Biomaterials, Vol. 32, Issue 23, pp. 5489-97, (2011) (PubMed).

Details zu beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Andere Bezeichnung: Amyloid beta (Abeta Produkte)

Synonyme: AAA antikoerper, ABETA antikoerper, ABPP antikoerper, AD1 antikoerper, APPI antikoerper, CTFgamma antikoerper, CVAP antikoerper, PN-II antikoerper, PN2 antikoerper, aaa antikoerper, abeta antikoerper, abpp antikoerper, ad1 antikoerper, appi antikoerper, ctfgamma antikoerper, cvap antikoerper, pn2 antikoerper, Abeta antikoerper, Abpp antikoerper, Adap antikoerper, Ag antikoerper, Cvap antikoerper, E030013M08Rik antikoerper, betaApp antikoerper, amyloid beta precursor protein antikoerper, amyloid beta (A4) precursor protein antikoerper, APP antikoerper, app antikoerper, App antikoerper

Hintergrund: Amyloid beta (Aβ or Abeta) is a peptide of 36 - 43 amino acids derived from the cleavage of APP (Amyloid Precursor Protein) by β- and γ-secretases. It is involved in Alzheimer´s disease, among other severe neurological disorders. It has been proved that the Aβ could be a crucial target for these important diseases treatment.

Pathways: Inflammasome