Hemoglobin Alpha 1 + 2 (HBA1,HBA2) (AA 2-142) Antikörper

Produktnummer ABIN5518839

Produktdetails

Target: Hemoglobin Alpha 1 + 2 (HBA1,HBA2)

Bindungsspezifität: AA 2-142

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Hemoglobin subunit alpha(HBA1|HBA2) detection. Tested with WB, IHC-P in Human,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Hemoglobin subunit alpha(HBA1|HBA2) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: hemoglobin subunit alpha 1/2
Protein Name: Hemoglobin subunit alpha

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Hemoglobin recombinant protein (Position: V2-R142). Human Hemoglobin shares 85.8% amino acid (aa) sequence identity with mouse Hemoglobin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Antigendetails

Target: Hemoglobin Alpha 1 + 2 (HBA1,HBA2)

Andere Bezeichnung: HBA1|HBA2 (HBA1,HBA2 Produkte)

Synonyme: HBAM antikoerper, Hba-a1 antikoerper, Hba-a2 antikoerper, CD31 antikoerper, HBH antikoerper, Hba antikoerper, Hba1 antikoerper, Hbat1 antikoerper, hemoglobin, alpha 1 antikoerper, hemoglobin subunit alpha 1 antikoerper, hemoglobin alpha, adult chain 1 antikoerper, Hba1 antikoerper, HBA1 antikoerper, Hba-a1 antikoerper

Hintergrund: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97 % of the total hemoglobin, alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3 % of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1, some nondeletion alpha thalassemias have also been reported.

Synonyms: CD31 | HBA 1 | HBA 2 | HBA | HBA T3 | HBA-T2 | HBA1 | HBA2 | Alpha-globin | P69905

Gen-ID: 3039, 3040

UniProt: P69905