CHD2 Antikörper (AA 1124-1351)

Produktnummer ABIN5518901

Produktdetails anti-CHD2 Antikörper

Target: CHD2 (Chromodomain Helicase DNA Binding Protein 2 (CHD2))

Bindungsspezifität: AA 1124-1351

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Chromodomain-helicase-DNA-binding protein 2(CHD2) detection. Tested with WB, IHC-P in Human,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Chromodomain-helicase-DNA-binding protein 2(CHD2) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: chromodomain helicase DNA binding protein 2
Protein Name: Chromodomain-helicase-DNA-binding protein 2

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human CHD2 recombinant protein (Position: R1124-K1351). Human CHD2 shares 97.4% amino acid (aa) sequence identity with mouse CHD2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu CHD2

Target: CHD2 (Chromodomain Helicase DNA Binding Protein 2 (CHD2))

Andere Bezeichnung: CHD2 (CHD2 Produkte)

Synonyme: CHD2 antikoerper, MGC148727 antikoerper, MGC159952 antikoerper, DKFZp469E1718 antikoerper, EEOC antikoerper, 2810013C04Rik antikoerper, 2810040A01Rik antikoerper, 5630401D06Rik antikoerper, AI851092 antikoerper, BC029703 antikoerper, microRNA 3175 antikoerper, chromodomain helicase DNA binding protein 2 antikoerper, MIR3175 antikoerper, CHD2 antikoerper, Chd2 antikoerper

Hintergrund: Chromodomain-helicase-DNA-binding protein 2 is an enzyme that in humans is encoded by the CHD2 gene. The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 catalyzes the assembly of chromatin into periodic arrays, and the N-terminal region of CHD2, which contains tandem chromodomains, serves an auto-inhibitory role in both the DNA-binding and ATPase activities of CHD2.

Synonyms: Chromodomain-helicase-DNA-binding protein 2, CHD-2, ATP-dependent helicase CHD2, CHD2

Gen-ID: 1106

UniProt: O14647