EGFR Antikörper (N-Term)

Produktnummer ABIN5518909

Der Kaninchen Polyklonal Anti-EGFR-Antikörper wurde für WB, IHC und FACS validiert. Er ist geeignet, EGFR in Proben von Human zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für EGFR Antikörper (N-Term) (ABIN5518909)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-EGFR Antikörper

Bindungsspezifität: AA 25-57, N-Term

Verwendungszweck: Anti-EGFR Antibody Picoband®

Sequenz: LEEKKVCQGT SNKLTQLGTF EDHFLSLQRM FNN

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-EGFR Antibody Picoband® (ABIN5518909). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human EGFR, different from the related mouse sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Herbst RS (2004). "Review of epidermal growth factor receptor biology". Int. J. Radiat. Oncol. Biol. Phys. 59 (2 Suppl): 21-6. 2. Kuan CT, Wikstrand CJ, Bigner DD (June 2001). "EGF mutant receptor vIII as a molecular target in cancer therapy". Endocr. Relat. Cancer 8 (2): 83-96. 3. Wang, K., Yamamoto, H., Chin, J. R., Werb, Z., Vu, T. H.: Epidermal growth factor receptor-deficient mice have delayed primary endochondral ossification because of defective osteoclast recruitment. J. Biol. Chem. 279: 53848-53856, 2004.

Kommentare:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-EGFR Antikörper (ABIN5518909)

Hao, Yang, Ding, Guo, Zhu, Ji, Zhou, Wu: "Paeoniflorin Potentiates the Inhibitory Effects of Erlotinib in Pancreatic Cancer Cell Lines by Reducing ErbB3 Phosphorylation." in: Scientific reports, Vol. 6, pp. 32809, (2018) (PubMed).

Shangguan, Jiang, Pan, Xiao, Tan, Tie, Qin, Deng, Chen, Wang: "Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats." in: Cell death & disease, Vol. 8, Issue 10, pp. e3157, (2018) (PubMed).

Cheng, Li, Liu, Cheng, Ma, Qiu: "YC-1 exerts inhibitory effects on MDA-MB-468 breast cancer cells by targeting EGFR in vitro and in vivo under normoxic condition." in: Chinese journal of cancer, Vol. 31, Issue 5, pp. 248-56, (2013) (PubMed).

Salenius, Haapanen, Harju, Jokela, Riekkinen: "Late carotid restenosis: aetiologic factors for recurrent carotid artery stenosis during long-term follow-up." in: European journal of vascular surgery, Vol. 3, Issue 3, pp. 271-7, (1989) (PubMed).

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR

Hintergrund:

Synonyms: Epidermal growth factor receptor,2.7.10.1,Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1,EGFR,ERBB, ERBB1, HER1,

Tissue Specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. .

Background: The breakpoint cluster region protein (BCR) is a protein that in humans is encoded by the BCR gene. A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 175 kDa

Gen-ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine