IRS4 Antikörper (AA 633-647)

Produktnummer ABIN5539610

Produktdetails anti-IRS4 Antikörper

Target: IRS4 (Insulin Receptor Substrate 4 (IRS4))

Bindungsspezifität: AA 633-647

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IRS4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Verwendungszweck: Irs4 (mouse aa633-647)

Sequenz: DRGTKERKEA KEVRD

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 150 kDa band observed in Mouse Heart lysates (calculated MW of 130 kDa according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 20

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25,14:831. PMID: 24274089.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-IRS4 Antikörper (ABIN5539610)

Ikink, Boer, Bakker, Hilkens: "IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation." in: Nature communications, Vol. 7, pp. 13567, (2018) (PubMed).

Details zu IRS4

Target: IRS4 (Insulin Receptor Substrate 4 (IRS4))

Andere Bezeichnung: Irs4 (IRS4 Produkte)

Synonyme: IRS4 antikoerper, irs-4 antikoerper, py160 antikoerper, IRS-4 antikoerper, PY160 antikoerper, irs2 antikoerper, irs2-a antikoerper, insulin receptor substrate 4 antikoerper, insulin receptor substrate 4 S homeolog antikoerper, IRS4 antikoerper, irs4 antikoerper, Irs4 antikoerper, irs4.S antikoerper

Hintergrund: Irs4, insulin receptor substrate 4, IRS-4, RP23-415G1.3, phosphoprotein of 160 kDa, pp160

Gen-ID: 16370, 315350

NCBI Accession: NP_034702