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C5AR1 Antikörper (C-Term)

Name: C5AR1 Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN5539670
Price: 779.48 EUR
Availability: InStock

Verified C5AR1 Reaktivität: Human WB, FACS, ELISA, IHC, IF Wirt: Ziege Polyclonal unconjugated

ABIN5539670 (0. (C5AR1 Antikörper (C-Term))

(ABIN5539670) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody. (C5AR1 Antikörper (C-Term))

IHC

ABIN5539670 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. (C5AR1 Antikörper (C-Term))

ABIN5539670 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0. (C5AR1 Antikörper (C-Term))

FACS

(ABIN5539670) (6 μg/mL) staining of paraffin embedded Human Spleen. (C5AR1 Antikörper (C-Term))

IHC

5 Abbildungen

Produktnummer ABIN5539670

Datenblatt (PDF)

Target Alle C5AR1 Antikörper anzeigen

C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
Bindungsspezifität
15

13

7

7

7

7

7

6

6

5

5

5

5

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
C-Term
Reaktivität
111

23

18

14

12

8

4

4

4

1

1

1

1

1

1
Human
Wirt
78

32

21

1
Ziege
Passende Sekundärantikörper
Klonalität
78

49

2
Polyklonal
Konjugat
60

12

10

10

6

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser C5AR1 Antikörper ist unkonjugiert
Applikation
70

63

39

20

15

14

13

13

8

8

6

2

2

2

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck

C5AR1/CD88

Sequenz

RESKSFTRST VDT

Kreuzreaktivität

Human

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Isotyp

IgG
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody
C5AR1 Reaktivität: Human, Rind (Kuh), Kaninchen, Frettchen WB, FACS, IHC (p), IP Wirt: Maus Monoclonal S5-1 unconjugated

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 250-350) antibody
C5AR1 Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibody
C5AR1 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 4E2 unconjugated

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-36) antibody
C5AR1 Reaktivität: Human WB, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (FITC)
C5AR1 Reaktivität: Human, Rind (Kuh), Kaninchen, Frettchen FACS Wirt: Maus Monoclonal S5-1 FITC

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 175-201) antibody
C5AR1 Reaktivität: Human WB, FACS Wirt: Kaninchen Polyclonal RB24276 unconjugated

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (APC)
C5AR1 Reaktivität: Human, Rind (Kuh), Kaninchen, Frettchen FACS Wirt: Maus Monoclonal S5-1 APC

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (PE)
C5AR1 Reaktivität: Human, Rind (Kuh), Kaninchen, Frettchen FACS Wirt: Maus Monoclonal S5-1 PE

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibody
C5AR1 Reaktivität: Human WB, PLA Wirt: Kaninchen Polyclonal unconjugated

anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibody
C5AR1 Reaktivität: Human WB, FACS Wirt: Maus Polyclonal unconjugated

Applikationshinweise

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx 38 kDa band observed in lysates of cell line HepG2 (calculated MW of 39.3 kDa according to NP_001727.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/m

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Andere Bezeichnung

C5AR1 (C5AR1 Produkte)

Synonyme

C5A antikoerper, C5AR antikoerper, C5R1 antikoerper, CD88 antikoerper, C5aR antikoerper, C5r1 antikoerper, Cd88 antikoerper, D7Msu1 antikoerper, GPR77 antikoerper, complement C5a receptor 1 antikoerper, complement component 5a receptor 1 antikoerper, C5AR1 antikoerper, C5ar1 antikoerper, c5ar1 antikoerper

Hintergrund

C5AR1, C5A, C5AR, C5A-R, C5R1, CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin receptor, Complement component 5 receptor 1, Complement component 5a receptor 1, Anaphylatoxin receptor

Gen-ID

728, 113959

NCBI Accession

NP_001727

Pathways

Komplementsystem