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beta-Galactosidase Tag Antikörper (HRP)

WB, EIA, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal HRP
Produktnummer ABIN5541543
  • Target Alle beta-Galactosidase Tag Produkte
    beta-Galactosidase Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 4
    • 1
    • 1
    Kaninchen
    Klonalität
    • 5
    • 1
    Polyklonal
    Konjugat
    • 4
    • 1
    • 1
    Dieser beta-Galactosidase Tag Antikörper ist konjugiert mit HRP
    Applikation
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    This antibody detects Beta-galactosidase [ E. coli ]. Cross reactivity against Beta-galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, and anti-rabbit serum as well as purified and partially purified Beta-galactosidase [E.coli].
    Aufreinigung
    Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
    Immunogen
    Beta-galactosidase isolated from E.coli
  • Applikationshinweise
    Suitable for Immunoblotting (Western or dot blot), ELISA, Immunofluorescence microscopy, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Western blot : 1/5,000-1/10,000. A 1/5,000 dilution has been reported to be successful for staining by Immunoblot of Beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus). ELISA : 1/10,000. Immunohistochemistry : 1/1,500. The antibody recognizes both Frozen tissue sections, Paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis:A 1/1,500 dilution has been reported to detect Beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 μm) to produce free-floating sections. A 1/5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1/10,000 dilution was noted to show no background and to be suitable for double labeling experiments). A 1/5,000 dilution has been reported to be successful for staining brain sections from transgenic mice expressing nuclear Beta-galactosidase when assayed by Immunofluorescence microscopy .
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    beta-Galactosidase Tag
    Abstract
    beta-Galactosidase Tag Produkte
    Substanzklasse
    Tag
    UniProt
    P00722
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