This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.
Aufreinigung
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Western blot : 1/1,000-1/5,000. Immunoprecipitation : 1/100. ELISA : 1/4,000-1/20,000. Immunohistochemistry on Paraffin (FFPE) or Frozen sections: 1/500-1/2,500.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Alcohol Dehydrogenases (ADH) are a group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In humans and many other animals, they serve to break down alcohols that otherwise are toxic, and they also participate in generation of useful aldehyde, ketone, or alcohol groups during biosynthesis of various metabolites.