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ANAPC10 Antikörper (AA 1-185)

Name: ANAPC10 Antikörper (AA 1-185)
Brand: antibodies-online
SKU: ABIN5542270
Price: 640.59 EUR
Availability: InStock

ANAPC10 Reaktivität: Human WB, IHC, ELISA, ICC, FACS Wirt: Maus Monoclonal 8F1D10 unconjugated

Western blot analysis using ANAPC10 mAb against human ANAPC10 (AA: 1-185) recombinant protein. (ANAPC10 Antikörper (AA 1-185))

Immunofluorescence analysis of Hela cells using ANAPC10 mouse mAb. (ANAPC10 Antikörper (AA 1-185))

Immunofluorescence analysis of Hela cells using ANAPC10 mouse mAb (green). (ANAPC10 Antikörper (AA 1-185))

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ANAPC10 mouse mAb with DAB staining. (ANAPC10 Antikörper (AA 1-185))

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (ANAPC10 Antikörper (AA 1-185))

ELISA

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ANAPC10 mouse mAb with DAB staining. (ANAPC10 Antikörper (AA 1-185))

IHC

Western blot analysis using ANAPC10 mAb against HEK293 (1) and ANAPC10 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate. (ANAPC10 Antikörper (AA 1-185))

Western blot analysis using ANAPC10 mouse mAb against Hela (1), MCF-7 (2), SK-Br-3 (3), A431 (4), HEK293 (5), A549 (6), and SPC-A-1 (7) cell lysate. (ANAPC10 Antikörper (AA 1-185))

Flow cytometric analysis of Hela cells using ANAPC10 mouse mAb (green) and negative control (red). (ANAPC10 Antikörper (AA 1-185))

FACS

9 Abbildungen

Produktnummer ABIN5542270

Datenblatt (PDF)

Target Alle ANAPC10 Antikörper anzeigen

ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))
Bindungsspezifität
15

6

4

1

1

1

1
AA 1-185
Reaktivität
23

20

6

2

2

2

2

2

1

1

1

1

1

1
Human
Wirt
35

3
Maus
Passende Sekundärantikörper
Klonalität
36

1
Monoklonal
Konjugat
17

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser ANAPC10 Antikörper ist unkonjugiert
Applikation
29

13

13

8

5

4

3

3

3

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Verwendungszweck

ANAPC10 Antibody

Aufreinigung

Purified antibody

Immunogen

Purified recombinant fragment of human ANAPC10 (AA: 1-185) expressed in E. Coli.

Klon

8F1D10

Isotyp

IgG1
anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 Reaktivität: Human WB Wirt: Kaninchen Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 Reaktivität: Human WB, IP Wirt: Kaninchen Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 Reaktivität: Human WB, IF Wirt: Maus Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (N-Term) antibody
ANAPC10 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Fledermaus, Huhn, Hamster, Affe, Schwein WB Wirt: Kaninchen Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (N-Term) antibody
ANAPC10 Reaktivität: Human, Maus, Ratte WB Wirt: Kaninchen Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (N-Term) antibody
ANAPC10 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Zebrafisch (Danio rerio) WB Wirt: Kaninchen Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody
ANAPC10 Reaktivität: Ratte WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (Biotin)
ANAPC10 Reaktivität: Ratte WB, ELISA, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal Biotin

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (AbBy Fluor® 350)
ANAPC10 Reaktivität: Ratte WB, IF (cc), IF (p) Wirt: Kaninchen Polyclonal AbBy Fluor® 350

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (AbBy Fluor® 488)
ANAPC10 Reaktivität: Ratte WB, IF (cc), IF (p) Wirt: Kaninchen Polyclonal AbBy Fluor® 488

Applikationshinweise

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Andere Bezeichnung

ANAPC10 (ANAPC10 Produkte)

Hintergrund

Description: ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1, MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1, MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1, MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).
Aliases: DOC1, APC10

Molekulargewicht

21.3kDa

Gen-ID

10393

HGNC

3417

UniProt

Q9UM13

Pathways

M Phase