Aldehyde Dehydrogenase Antikörper (HRP)

Produktnummer ABIN5596684

Produktdetails anti-Aldehyde Dehydrogenase Antikörper (HRP)

Target: Aldehyde Dehydrogenase (ALDH)

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aldehyde Dehydrogenase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Aldehyde Dehydrogenase from other sources is unknown.

Aufreinigung: Aldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast].

Immunogen:

Immunogen: Aldehyde Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Aldehyde dehydrogenase has been assayed against 1.0 μg of Aldehyde Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Aldehyde Dehydrogenase

Target: Aldehyde Dehydrogenase (ALDH)

Andere Bezeichnung: Aldehyde Dehydrogenase (ALDH Produkte)

Hintergrund:

Synonyms: Aldehyde dehydrogenase [NAD(P)+] 1 ALD2, ALD5

Background: Aldehyde dehydrogenase are a group of enzymes that catalyse the oxidation (dehydrogenation) of aldehydes. Aldehyde dehydrogenase is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, which leave the liver and are metabolized by the body's muscle and heart. ALDH2 also plays a crucial role in maintaining low blood levels of acetaldehyde during alcohol oxidation.

Gene Name: ALD2, ALD5

Gen-ID: 855206

UniProt: P47771