Calf Intestinal Alkaline Phosphatase (CIAP) Antikörper (HRP)

Produktnummer ABIN5596686

Hersteller Produkt- Nr.: 200-1335-0100

Produktdetails

Target: Calf Intestinal Alkaline Phosphatase (CIAP)

Reaktivität: Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Hersteller Produkt- Nr.: 200-1335-0100

Hersteller: Rockland

Verwendungszweck: Alkaline Phosphatase Antibody HRP Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].

Produktmerkmale: Synonyms: goat anti-Alkaline Phosphatase Antibody HRP Conjugation, Peroxidase Conjugation goat anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Aufreinigung: Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Alkaline Phosphatase [Calf Intestine]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Alkaline Phosphatase peroxidase has been tested by dot blot and is functional in ELISA, Western blot, and Immunohistochemical assays. Optimization should be reached by researcher.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: Calf Intestinal Alkaline Phosphatase (CIAP)

Andere Bezeichnung: Calf Intenstinal Alkaline Phosphatase (CIAP Produkte)

Hintergrund: Background: Alkaline Phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. Humans and most other mammals contain the following alkaline phosphatase isozymes: ALPI - intestinal, ALPL - tissue non-specific (liver/bone/kidney), ALPP - placental (Regan isozyme). Elevated ALP indicates that there could be active bone formation occurring as ALP is a byproduct of osteoblast activity (such as the case in Paget's disease of bone). Levels are also elevated in people with untreated Coeliac Disease. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).

Gen-ID: 280993

UniProt: P19111