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Choline Oxidase Antikörper (HRP)

Reaktivität: Alcaligenes WB, ELISA, IP Wirt: Ziege Polyclonal HRP
Produktnummer ABIN5596705
  • Target Alle Choline Oxidase Produkte
    Choline Oxidase
    Reaktivität
    • 7
    • 5
    Alcaligenes
    Wirt
    • 7
    • 5
    Ziege
    Klonalität
    • 12
    Polyklonal
    Konjugat
    • 6
    • 5
    • 1
    Dieser Choline Oxidase Antikörper ist konjugiert mit HRP
    Applikation
    • 11
    • 10
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Kreuzreaktivität (Details)
    Cross reactivity against Choline Oxidase from other sources is unknown.
    Aufreinigung
    Anti-Choline oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].
    Immunogen

    Immunogen: Choline Oxidase [Alcaligenes species]

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise

    Application Note: Anti-Choline oxidase has been assayed against 1.0 μg of Choline Oxidase [Alcaligenes species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.

    Western Blot Dilution: 1:500 - 1:1,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:1,000 - 1:2,500

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Konservierungsmittel
    Gentamicin sulfate
    Vorsichtsmaßnahmen
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    Choline Oxidase
    Abstract
    Choline Oxidase Produkte
    Synonyme
    Afu8g04090 antikoerper, choline oxidase antikoerper, choline oxidase (CodA) antikoerper, SAV_6946 antikoerper, AFUA_8G04090 antikoerper, AAur_0512 antikoerper, Bfae_22540 antikoerper, BDBG_07023 antikoerper, codA antikoerper, Mspyr1_24000 antikoerper, TERG_03224 antikoerper
    Hintergrund

    Synonyms: codA antibody EC=1.1.3.17

    Background: Choline oxidase is an enzyme that catalyzes the chemical reaction (choline + O2 <=> betaine aldehyde + H2O2). Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. Choline oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycine, serine, and threonine metabolism.

    Gene Name: CHOX_ALCSP

    Gen-ID
    116367
    UniProt
    P16101
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