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Elastase Antikörper (HRP)

Reaktivität: Schwein ELISA, WB, IHC Wirt: Kaninchen Polyclonal HRP
Produktnummer ABIN5596709
  • Target Alle Elastase Antikörper anzeigen
    Elastase
    Reaktivität
    • 13
    • 10
    • 2
    Schwein
    Wirt
    • 18
    • 5
    • 1
    • 1
    Kaninchen
    Klonalität
    • 20
    • 5
    Polyklonal
    Konjugat
    • 17
    • 4
    • 1
    • 1
    • 1
    • 1
    Dieser Elastase Antikörper ist konjugiert mit HRP
    Applikation
    • 14
    • 11
    • 7
    • 5
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Kreuzreaktivität (Details)
    Cross reactivity against Elastase from other tissues and species may occur but have not been specifically determined.
    Aufreinigung
    Elastase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas].
    Immunogen

    Immunogen: Elastase [Porcine Pancreas]

    Immunogen Type: Native Protein

    Isotyp
    IgG
    Top Product
    Discover our top product Elastase Primärantikörper
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Elastase has been assayed against 1.0 μg of Elastase [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:5,000 - 1:20,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Konservierungsmittel
    Gentamicin sulfate
    Vorsichtsmaßnahmen
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    Elastase
    Abstract
    Elastase Produkte
    Hintergrund

    Synonyms: Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

    Background: Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. Elastase breaks down elastin, an elastic fiber that, together with collagen, determines the mechanical properties of connective tissue. Elastase also has the important immunological role of breaking down Shigella virulence factors. Elastase is responsible for pulminary emphysema, cyclic hematopoeiesis, and blistering in bullous pemphigoid.

    Gene Name: CELA1

    Gen-ID
    396766
    UniProt
    P00772
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