Ferritin Antikörper (HRP)

Produktnummer ABIN5596710

Produktdetails anti-Ferritin Antikörper (HRP)

Target: Ferritin (FE)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ferritin Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Kreuzreaktivität (Details): Cross reactivity against Ferritin from other sources is unknown.

Aufreinigung: Anti-Ferritin (Human Spleen) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Immunogen:

Immunogen: Ferritin [Human Spleen]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Ferritin (Human Spleen) Antibody has been assayed against 1.0 μg of Ferritin [Human Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Ferritin (Human Spleen) Antibody.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Kommentare:

User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Ferritin

Target: Ferritin (FE)

Andere Bezeichnung: Ferritin (FE Produkte)

Synonyme: 1HCH antikoerper, BEST:LD36673 antikoerper, CG2216 antikoerper, Dmel\\CG2216 antikoerper, FER1 antikoerper, FER1HCH antikoerper, Fer-L antikoerper, Fer1 antikoerper, Fer1HC antikoerper, FerHCH antikoerper, HCH antikoerper, T21 antikoerper, T9 antikoerper, fer1 antikoerper, fer1hch antikoerper, l(3)00451 antikoerper, l(3)j10B4 antikoerper, SFerH-1 antikoerper, SOF-35 antikoerper, SOF-5L antikoerper, NV13011 antikoerper, ACP1 antikoerper, F8A5.24 antikoerper, F8A5_24 antikoerper, Ferritin 1 heavy chain homologue antikoerper, ferritin light chain antikoerper, ferritin antikoerper, chromosome 26 open reading frame, human C10orf131 antikoerper, hyaluronan and proteoglycan link protein 2 antikoerper, NAD(P)-linked oxidoreductase superfamily protein antikoerper, Fer1HCH antikoerper, H-1 antikoerper, YPDSF_1340 antikoerper, ftnA antikoerper, Ft antikoerper, C26H10orf131 antikoerper, HAPLN2 antikoerper, AT1G60730 antikoerper

Hintergrund:

Synonyms: Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

Background: Ferritin is a intracellular protein that directly stores iron and regulates its release, specifically acting as a buffer for iron in humans. Ferritin is composed of 24 subunits and comes in two types, H and L. The H subunit detoxifies iron while the L subunit deals with long term storage of iron. Depending on the concentration of ferritin in the blood, different disorders and diseases could be present in an individual. Anti-Ferritin (Human Spleen) Antibody is ideal for investigators in Cancer, Immunology, and Cardiovascular Research.

Gen-ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis