LMW HCP Antikörper

Produktnummer ABIN5596745

Produktdetails anti-LMW HCP Antikörper

Target: LMW HCP (LMW Host Cell Proteins (LMW HCP))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE), Western Blotting (WB)

Verwendungszweck: Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli.

Produktmerkmale: Synonyms: rabbit anti-E.Coli Host Cell Protein antibody, rabbit anti-HCP antibody, rabbit anti-Low molecular Weight E.Coli Host Cell Proteins Antibody, Anti-Host Cell Protein, Low molecular weight E.Coli HCP proteins antibody

Aufreinigung: LMW Host Cell Protein A purified antibody is directed against low molecular weight E.coli Host Cell Proteins.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-LMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli (BL21).

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-LMW Host Cell Proteins Antibody detects over 270 E.coli LMW proteins and has been tested by 2D-Page, western blotting, and ELISA.

Western Blot Dilution: 1:500-1:1000

ELISA Dilution: 1:1000 - 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.29 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu LMW HCP

Target: LMW HCP (LMW Host Cell Proteins (LMW HCP))

Andere Bezeichnung: LMW E.Coli Host Cell Protein

Hintergrund: Background: Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli. This antibody has been analyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.